Making use of single-molecule optical and AFM imaging and force-spectroscopy procedures, we search for to unravel the mechanistic facets of the structure and dynamics of chromosomes. Our research pursuits contain:
But how do morality, the dignity from the individual, my knowledge of your indicating of daily life, stick to in the motion of atoms? A purely naturalistic viewpoint fails here; a Christian point of view, I believe, is a way more realistic method to interpret these components of fact.’
Being a individual, I have the biblical assignment to check out and use everything in development for the benefit of my neighbour. I tend not to expertise any conflict between religion and science. Quite the opposite, my religion stimulates me to carry out science, being open-minded on earth.’
Non-equilibrium folding of individual DNA molecules recaptured as much as a thousand situations within a sound state nanopore
That could be a aspiration of mine and we set to operate which has a delightful dose of naivety. This really is a thing that will probably produce amazing results in the extended term, but that does not make any difference. You should not only want to work to the concerns of tomorrow, and also on the queries with the working day right after tomorrow.’
Experimental stage diagram of negatively supercoiled DNA calculated by magnetic tweezers and fluorescence,
What distinguishes biology from chemistry is the fact these elements alongside one another make up a residing procedure. The key of reported daily life, I think, lies while in the interactions, the interaction of Individuals molecules. The massive issue in regards to the origin of life continues to be unanswered. How did we at any time evolve from easy molecules in the extremely intricate systems we see nowadays? We do not know. I would like to operate on These types of large, open up questions.’
Finding out DNA loop extrusion by SMC proteins for chromosome architecture with single-molecule assays.
2003, demonstrated the main biosensors built away from a carbon nanotube; settled the construction and system of DNA repair proteins; and discovery of a new system for fabricating stable-condition nanopores for DNA translocation
Dekker started his exploration on solitary carbon nanotubes in 1993 when he set up a completely new line of investigation to study electrical transportation by means of solitary natural and organic molecules amongst nanoelectrodes. In 1996 a breakthrough was realized with carbon nanotubes. This was obtained within a collaboration Using the team of Nobel laureate Richard Smalley. STM and nanolithography methods were being accustomed to reveal that these nanotubes are quantum wires at The only-molecule amount, with exceptional Actual physical Homes.
Given that 2000 he moved to single-molecule biophysics and nanobiology, with exploration from scientific studies of DNA loop extrusion and supercoiling to DNA translocation through nanopores. Additional not too long ago his research has focused on finding out chromatin structure and mobile division with bacteria on chip, when he can also be aiming to in the end Make synthetic cells from The underside up.
Cees Dekker is Distinguished University Professor at TU Delft. Educated as being a physicist, he pioneered nanotechnology with numerous discoveries on carbon nanotubes within the 1990s. Since 2000, he moved to one-molecule biophysics and nanobiology. ceesdekker.net His present-day investigate concentrates on (i) nanopores for protein sequencing; (ii) chromosome framework, particularly SMC motor protein; and (iii) building a living artificial mobile from lifeless molecules. Dekker is undoubtedly an elected member on the KNAW and fellow on the APS as well as the IOP.
Publisher’s Be aware Springer Character remains neutral with regards to jurisdictional promises in released maps and institutional affiliations.
2013, managed shaping of live bacterial cells into arbitrary designs; and discovery of spontaneous fluctuations during the handedness of histone tetrasomes